| Primer | Species | LP | RP | Tml | Tmr | Length | Score ⇅ | On-target ⇅ | RI genome sensitivity ⇅ |
|---|---|---|---|---|---|---|---|---|---|
| Primer 21 | Burkholderia pseudomallei | AAGCACGCTGGCGAAGAT | TCGTTTCGGTCACGGTGT | 60.05 | 59.19 | 257 | 67 |
100.00%
|
50.00%
|
| Primer 22 | Burkholderia pseudomallei | AAGCACGCTGGCGAAGAT | CTCGTTTCGGTCACGGTGT | 60.05 | 60.30 | 258 | 67 |
100.00%
|
50.00%
|
| Primer 23 | Burkholderia pseudomallei | AATGACCGTCAACCTGCCC | TGCATGTACGGGAAGAGCG | 60.30 | 60.15 | 216 | 66 |
100.00%
|
50.00%
|
| Primer 24 | Burkholderia pseudomallei | AAGCACGCTGGCGAAGAT | TTTCGGTCACGGTGTAGCC | 60.05 | 60.01 | 254 | 64 |
100.00%
|
50.00%
|
| Primer 25 | Burkholderia pseudomallei | TGCTAGACGACAAGGCGTG | AGTTGAAGCGCACGGTGT | 60.08 | 60.20 | 279 | 64 |
100.00%
|
50.00%
|
| Primer 26 | Burkholderia pseudomallei | TCGACGAGAAGCGCAACA | TTCGAGCCGTGCATGCA | 59.67 | 60.01 | 208 | 63 |
100.00%
|
50.00%
|
| Primer 27 | Burkholderia pseudomallei | TGATCGGCTGGGATTGCA | CGCGACCGTTGCAAAACA | 59.00 | 59.98 | 152 | 63 |
100.00%
|
50.00%
|
| Primer 28 | Burkholderia pseudomallei | CTGATCGGCTGGGATTGCA | CGCGACCGTTGCAAAACA | 60.15 | 59.98 | 153 | 63 |
100.00%
|
50.00%
|
| Primer 29 | Burkholderia pseudomallei | TGCTGATCGGCTGGGATTG | CGCGACCGTTGCAAAACA | 60.15 | 59.98 | 155 | 63 |
100.00%
|
50.00%
|
| Primer 30 | Burkholderia pseudomallei | ATGACGACCCACCGTTTCG | CGTTGCGCTTTCGTGGAA | 60.37 | 59.37 | 186 | 61 |
100.00%
|
50.00%
|